Fig 3-3.2.13: Comparison of symmetric PCR, standard asymmetric PCR, and LATE-PCR (respectively from top to bottom, Reference : Sanchez et al 2004 )

Symmetric PCR reaches a plateau around 50 cycles and shows considerable variability in kinetics with a simultaneous large end-point range. While, conventional asymmetric PCR is flawed by delayed linear kinetics and lower end-point signals. Thus, LATE-PCR only shows the efficiency in linear kinetics for over than 80 cycles.