Module 5 : GENE TRANSFER TECHNIQUES

Lecture 5 : Chloroplast Transformation

Marker

Selection

Organism

Photosynthesis

atpB

Photo autotrophy

Chlamydomonas

tscA

Photo autotrophy

Chlamydomonas

psaA/B

Photo autotrophy

Chlamydomonas

petB

Photo autotrophy

Chlamydomonas

rbcL

Photo autotrophy

Tobacco

Drug resistance

rrnS

Spectinomycin, Streptomycin

Chlamydomonas,
Tobacco, Tomato

rrnL

Erythromycin

Chlamydomonas

nptII

Kanamycin

Tobacco (low transformation efficiency), Cotton

aphA-6

Kanamycin, Amikacin

Chlamydomonas,
Tobacco, Cotton

aad (preferred for chloroplast transformation)

Trimethoprim, Spectinomycin, Streptomycin, Sulphonamides

Solanum tuberosum (Potato)

ble

Bleomycin

Tobacco

dhfr

Methotraxate

Rice

hpt

Hygromycin

Rice

gat

Gentamycin

Tobacco

Herbicide resistance

psbA

DCMU, metribuzin

Chlamydomonas

bar (not good enough for transformation) and pat

Phosphinothricin

Tobacco

AHAS

Sulfometuron methyl

Porphyridium sp.

EPSP

Glyphosate

Tobacco

HPPD

Diketonitrile

Tobacco

Csr 1-1

Chlorsulphuron

Rice

dhps

Sulphonamides

Tobacco

Metabolism

BADH

Betaine aldehyde

Tobacco

coda

5-fluorocytosine

Tobacco

ARG9

Arg autotrophy

Chlamydomonas

ASA2

Trp analogues

Tobacco

Table 5-5.7.1. Various selectable markers used for chloroplast transformation