Figure 8-1.7.2.7: Mechanism of gene transfer by sleeping beauty transposition

Adapted and modified from:

http://www.discoverygenomics.net/pdf/SBT_ProlongedExpressions.pdf

 

•  RNA-DNA Chimera

This RNA- DNA chimera is also known as chimeroplast used to correct point mutations by mismatch repair. This 68 nucleotides long double stranded nucleic acid molecule comprises of one strand DNA and another strand consisting of two 10 nucleotides long 2'-O methyl RNA stretch separated by a 5 nucleotide long DNA stretch as shown in Figure 8-1.7.2.8. The pentameric chimeric DNA carries the mismatch and the other DNA strand has its complementary bases. Additionally this RNA-DNA chimera consists of two hairpin loop and a GC clamp.