Optical microscopes:

Light Microscope: Magnification

Magnification of the Microscope

M of Microscope = M of Objective x M of Eyepiece x M of Intermediate Factor
• For Example:
Objective = 60 x
Eyepiece = 10 x
Intermediate Factor = 1 x

Overall M turns out to be 600 x

The magnification of the compound microscope is given as M =m_o M_e

Where, M_e is angular magnification of eyepiece given as 25/f_e and m_o is linear magnification of objective defined as -( L / f_o). f_o and f_e are the focal lengths of objective and eye-piece, respectively as given in Figure 10.03.

Figure 10.03: Schematic of viewing image using light microscope.

Light Microscope: Types

There are many types of light microscopes for imaging purposes:

• Bright-field microscope

• Dark-field microscope

• Phase-contrast microscope

• Fluorescence microscope