Figure 28.5: Relationship between number of distribution planes (N) and peak width.

Different components of chromatography system: The different components of a chromatography system are given in Figure 28.6. It has following components:

1. Reservoir: One or two reservoir for mobile phase (buffer).

2. Pump: One or two pump to flow the buffer from reservoir. Different types of pumps are used in chromatography system, mostly based on the pressure level required to perform chromatography. A pump is chosen as per the pressure required to run the mobile phase. Based on the pressure level, liquid chromatography can be classified into the following categories :

Low Pressure Liquid Chromatography: Pressure limit less than 5 Bar.

Medium Pressure Liquid Chromatography : Intermediate pressure limit (6-50 bar).