Figure 28.3: Seperation of two molecules on a column.

Chromatogram: The plot of elution volume along with the absorbance is known as chromatogram as given in Figure 28.4. The volume or time it takes for a analyte to come out from the column is known as retention volume or time. The chromatogram may have separate peaks (A and B) or peaks (C and D) with overlapping base, these peaks are called fused peaks.

Figure 28.4: A typical chromatogram.