Module 7 : Microbial Molecular Biology and Genetics

Lecture 8 : Mutations and their Chemical Basis, Detection and Isolation of Mutants

 

Study Questions:

  1. 1. Define: Mutation, Mutagen, and Mutant

    2. 2. What are the different types of mutagens?

    3. What does chemical mutagen do, and example for it?

    4. How radiation causes changes in the DNA molecule. Explain?

    5. Define: Spontaneous mutation

    6. 5- Bromouracil is an analogue of……………….?

    7. Thymine dimer is caused by………………………….?

    8. What is silent mutation? Will it affect the protein synthesis?

    9. Explain: Missense, Nonsense and Frameshift mutation

    10. What will happen if the mutation occurs in so called non coding DNA? Justify your answer.

    11. List out the possible results of mutational effect on protein?

    12. Explain: chromosomal mutations

    13. What are the different types of chromosomal mutation?

    14. Explain: Deletion, Duplication, Inversion, and translocation.

    15. What is positive selection in mutant isolation? Explain how will you accomplish that?

    16. What is negative selection in mutant isolation? Explain how will you accomplish that?

    17. What do you mean by auxotrophic mutant?

    18. What is replica plating? How will you perform that experiment?