Module 7 : Microbial Molecular Biology and Genetics

Lecture 3 : The Genetic Code and the Gene Structure

 

Study Questions:

  1. 1. What is codon?

    2. One amino acid codes………………………. Nucleotides?

    3. Number of possible codons………………………….

    4. Define: Degenerate codon, Nonsense codon

    5. Could you recall all the amino acids and its corresponding triplets?

    6. Explain about overlapping and non-overlapping codon?

    7. What is the start codon for prokaryote and eukaryote?

    8. What are termination codon and their specific name?

    9. Is that nuclear DNA and Mitochondrial DNA genetic code same?

    10. Write down the characteristics of the Genetic code?

    11. Explain Wobble hypothesis?

    12. What is called as wobble base? Example……..?

    13. What is Unusual base? Example………..?

    14. Explain: Wobble pairing?

    15. Explain: Gene?

    16. Explain: Cistron, Intron and Exon?

    17. Whether the gene and cistron are same? Think about it……

    18. Does the prokaryotic gene has intervening sequence or the eukaryotic gene has the intervening sequence? Justify your answer.

    19. Explain: Monocistroninc mRNA and Polycistronic mRNA?

    20. Can you draw the structure of functional gene?

    21. Explain: Promoter, enhancer?

    22. Explain Operon? Give an example ………………….?

    23. Explain: transcription, transcriptome?

    24. Where is RNA Pol recognition site on the template strand ?

    25. Name the RNA Pol binding site on DNA and its location from start site?

    26. What is Shine-Dalgarno sequence?

    27. What is the first amino acid translated from matured mRNA?

    28. What is TATA box?

    29. How the gene is transcribed and terminated? Explain in detailed?