Study Questions:

1. 1. What is the mode of replication?

   2. What is sense and antisense strands?

   3. Explain origin of replication and where it will start at prokaryote?

   4. Role of Helicase, DnaA, Topoisomerase 1 and SSBPs protein replication of DNA?

   5. Replication in bacteria is unidirectional or bidirectional?

   6. What do you know about “ Theeta ” mode of replication?

   7. Explain: Replicon.

   8. How polymerization occurs in DNA molecule or how the bases are added to the growing strand?

   9. Why RNA priming is needed? Why can't DNA itself?

   10. What is the name of the enzyme which synthesizes RNA primers?

   11. Explain: Leading strand and lagging strand, Okazaki fragments.

   12. Name the enzyme responsible for DNA synthesis? And its subunits function?

   13. Define: Replisome

   14. Role of DNA polymerase I in replication?

   15. RNA primer removal from the lagging strand is accomplished by…………………..

   16. DNA Pol I have ………………. and ……………….. Activity

   17. Proof reading and DNA repair in done by which Polymerases? How they will do?

   18. Role of DNA Ligase enzyme in DNA synthesis?