Study Questions:

1. 1. Two major functions of DNA molecule?

2. What is Central Dogma? How it can be represented?

3. What is the number of RNA polymerase in prokaryote and eukaryote?

4. Write down the number of subunits which prokaryotic RNA Pol consists of?

5. What is Holoenzyme?

6. Explain briefly: Transcription initiation, elongation and termination?

7. What is the role of Sigma factor in transcription? And also the different types of sigma factor?

8. What is the difference between prokaryotic and eukaryotic transcription initiation?

9. TBP means…………………….?

10. Write down the elongation factors of prokaryote as well as eukaryote?

11. What do you mean by activator, enhancer, and repressor? Explain their role in transcription?

12. What is rho independent termination?

13. Does the rho dependent termination need any specific protein for its transcription termination activity? If so, explain briefly.

14. What is the difference between rho dependent and rho independent termination?

15. Explain about transcription termination in eukaryotes?

16. What is called CpG Island? Why it is important?

17. What do you mean by capping? Why it is so important?

18. Why RNA processing occurs in Eukaryotes? Why not in prokaryotes? Justify.

19. What is reverse transcription?

20. What is the function of reverse transcriptase?

21. Explain the role of Ribonuclease H?

22. What is function of telomerase? Where it is acting?

23. Can you able to recall the process of overall transcription?

24. What is the role of ribosome in protein synthesis? And what are the different sub units?

25. What is the role of tRNA?

26. Can you draw the structure of tRNA and mention its most important part?

27. Explain: translation initiation? How it is initiated?

28. Explain: translation elongation? How it is elongated?

29. What are the two different sites on the elongation sites?

30. Explain: translation termination? How it is terminated?

31. Draw the complete process of translation?