Answers

I. Choose the correct answer.

1. (B) Increment of absorbance when any material is exposed to UV light.

2. (C) Nucleic acids

3. (B) to amplify a stretch of nucleic acid sequences

4. (A) as a co-factor

5. (A) RNA strand is reverse transcribed into its complementary DNA

6. (C) Nested PCR

7. (D) Hydrogen bond

8. (A) DNA

9. (B) Hybridization process

10. (A) exposes 3′- OH termini and 5′-PO₄ termini

11. (A) substitution of puine base by pyrimidine or vice versa

12. (B) Dimerization of pyrimidine bases

II. Fill in the blanks with appropriate words and terminology.

1. UV absorbance 2. 215-230 nm and 280 nm 3. Hyperchromicity 4. 2, 7-diamino-10-ethyl-9-phenylphenanthridiniumbromide. 5. Kary Mullis and Taq polymerase 6. Annealing temperature 7. 3' end and 5'end 8. RNA strand and complementary DNA copy 9. Increases 10. Threshold cycle 11. E.M Southern 12. James Alwine, George Stark and David Kemp 13. Probe 14. Target. 15. Polynucleotide kinase 16. ³²P, ³³P,³⁵S or ³H. 17. Digoxigenin, 18. The planar structure and ds DNA 19. Transposons