I. Choose the correct answer.

1. What is Hyperchromaticity?

(A) Decrease of absorbance when any material is exposed to UV light.

(B)  Increment of absorbance when any material is exposed to UV light.

(C)  Decrease of melting temperature of DNA

(D) Increment of melting temperature of DNA.

2. Agarose gel is used to separate-

(A) DNA

(B)  RNA

(C)  Nucleic acids

(D) Protein

3. PCR is the process

(A) to amplify a stretch of amino acid sequences

(B)  to amplify a stretch of nucleic acid sequences

(C)  to cleave a stretch of long nucleotide sequences

(D) to amplify a stretch of deoxy ribo nucleotide sequences

4. Divalent cations are used in PCR reaction mixture:

(A) as a co-factor

(B)  as an inhibitor

(C)  as a catalyst

(D) none of the above

5. Reverse Transcription PCR is used to

(A)  RNA strand is reverse transcribed into its complementary DNA

(B)  amplify DNA sequence

(C)  quantify the copy number of nucleotide sequences.

(D)  All of the above.

6. ‘inner primers' and ‘outer primers' are used in

(A) Reverse transcription PCR

(B)  Quantitative PCR

(C)  Nested PCR

(D) Inverse PCR