IV. Answer the following short questions yourself.

Q1. What are the basic features to design primers for basic PCR reaction?

Q2. What are the different hybridization based methods to detect nucleic acids?

Q3. Briefly elucidate the process of colony PCR and its application in basic cloning process.

Q4. What are different types of in-situ hybridization process and describe comparison among them.

Q5. What are RNA probes? Describe one method to label RNA probe.

Q6. What are the basic processes involved in in-vivo site directed mutagenesis?

V. Answer the following long questions yourself.

Q1. Explain various methods which are involved in detection of nucleic acids.

Q2. Mention the different components used in PCR reaction and describe their role in detail

Q3. Describe the different types of PCR which are commonly used in molecular biology.

Q4. What are the various methods of nucleic acid hybridization process and briefly describe each process.

Q5. What are probes? Mention different types of probes and how it can be used in nucleic acid hybridization process.

Q6. What is mutagenesis process? Mention various types of mutagenesis process and briefly describe each process.