Figure 30.9 :Movement of Ribosome ot m RNA
Elongation process requires three elongation factors
EF-Tu |
It is G protein which binds to amino acyl t RNA and directs it to correct position at ribosome a site. |
EF-Ts |
Its main function is to regenerate to EF-Tu and hydrolysis of GTP |
EF-G |
It is also a G protein which mediates translocation. |
Process of chain elongation on ribosome: EF-TU promotes the entry of amino acyl t-RNA into the A site of 70 s ribosome. Firstly EF-TU bind to GTP and it activated the EF-TU GTP complex which bind t-RNA. When codon and anti-codon base pairing stabilised then hydrolysis of GTP occurs which converts into GDP and Pi, which helps in binding of aminoacyl t RNA to A site and after this EF-TU is released. EF-TS is catalysed the release of EF-TUGDP from ribosome and regenerate EF-TUGTP, Its main work is to recycle the EF-TU,given in Figure 30.10.
Figure 30.10 :Working of EF-TU