Module 4 : Molecular Cell Biology

Lecture 30 : Translation (Part-I)

 

Figure 30.9 :Movement of Ribosome ot m RNA

Elongation process requires three elongation factors


EF-Tu   

It is G protein which binds to amino acyl t RNA and directs it to correct position at ribosome a site.

EF-Ts   

Its main function is to regenerate to EF-Tu and hydrolysis of GTP

EF-G   

It is also a G protein which mediates translocation.

Process of chain elongation on ribosome: EF-TU promotes the entry of amino acyl t-RNA into the A site of 70 s ribosome. Firstly EF-TU bind to GTP and it activated the EF-TU  GTP complex which bind  t-RNA. When codon and anti-codon base pairing stabilised then hydrolysis of GTP occurs which converts into GDP and Pi, which helps in binding of aminoacyl t RNA to  A site and  after this EF-TU is released. EF-TS is catalysed the release of EF-TUGDP from ribosome and regenerate EF-TUGTP, Its main work is to recycle the EF-TU,given in Figure 30.10.

Figure 30.10 :Working of EF-TU