Module 3 : Microscopy techniques

Lecture 12 : Basics and Principles

Transmission Electron Microscope:

Instrumental Part:

Objective Lens:

• The objective lens (see Figure 12.06) forms an inverted initial image, which is subsequently magnified. In addition, in the back focal plane of the objective lens a diffraction pattern is formed. Hence, the objective aperture has to be utilized to select the components. Note that the objective lens usually would not provide a magnification of more than 50✗ and any magnification of the image is mainly done by using projector lenses.

Projector Lens:

• œMagnification in the electron microscope can be varied from few hundreds to several hundred thousands of times. œ

• This is achieved by varying the strength of the projector lenses and intermediate lenses as shown in Figure 12.06. œ


Figure 12.06: Schematic of lens system including projector lens in TEM.

• Note that all lenses need not (unnecessarily) be used at lower magnifications.

œThe introduction of each lens in the system for magnification changes the direction of final image (see arrow mark on the left side) on a fluorescent screen.