E. Observation: A typical observation of the drug treated parasite is given in the Figure 30.6.
Figure 30.6: Differences in the cellular morphology of healthy parasites vs. drug treated parasites
F. Results and calculation of IC50: The number of schizont containing RBCs were counted against each concentration. The schizont inhibition data from the in vitro in vitro schizont inhibition assays of the above compounds were fed into a specially pre-programmed excel sheet HN-NonLin available freely from (www.malaia.farch.net) . To determine the nature of action (parasitotatic/parasicidal), in 100 µl volume, 3 % haematocrit with 1 % parasites were exposed to trial compounds for 48 hours. After 48 hours, parasites were washed twice with complete media and again incubated for 48 hour in drug free media. Then smears were made and parasitemia has been determined microscopically.