Module 4 : Molecular Cloning-II

Lecture 21 : DNA Delivery in Host Cell (Part-II)

Quiz

Q1: What is the role of heat shock in the transformation of bacteria in CaCl2 method?

Q2: Why generation of spheroplast gives better transformation efficiency in yeast?

Q3: What are the draw back of calcium-phosphate method of DNA delivery in mammalian cells?

Q4: The process through which DNA-lipid complex is taken up by the mammalian cells?

Q5: What is the significance of a helper plasmid in virus mediated DNA delivery in mammalian cells?