DNA Delivery in mammalian cells- mammalian cell membrane surface chemistry, intracellular comparatmentization and uptake mechanism is different from the prokaryotic cells or yeast. Hence specialized methods have been developed to suit mammalian cells. There are 4 major strategies to deliver the DNA in mammalian cells:

1. Chemical transfection techniques- The principle behind the chemical transfection technique is to coat or complex the DNA with a polymeric compound to a reasonable size precipitate (Figure 21.1). It facilitates the interaction of the precipitate with the plasma membrane and uptake through endocytosis. There are multiple chemical compounds have been discovered which can be able to make complex and deliver DNA into the mammalian cell.

Figure 21.1: Mechanism of chemical method mediated DNA delivery in animal cells.