Module 1 : APPLICATIONS OF PLANT BIOTECHNOLOGY IN CROP IMPROVEMENT

Lecture 3 : Tissue Culture Media

 

Study Questions :

 

1.  What are some possible substitutes of agar as a medium matrix in plant tissue culture? Discuss their possible advantages and disadvantages?

2.  Expand the following:

(a) HEPA (b) PCPA (c) NOA (d) 2iP (f) TIBA (g) PCIB (h) NAA

3. Molecular weight of BAP is 225.2. What amount of BAP is required to make a 50ml stock of 10-3 M concentration?

4. Match the following:

Element
Function
i) Carbon a) Important constituent of the cell wall and enzyme cofactor
ii) Magnesium b) Part of cytochromes
iii) Iron c) Important enzyme cofactor and part of chlorophyll
iv) Calcium d) Common cellular component and form basic backbone of most biochemical.