Module 1 : APPLICATIONS OF PLANT BIOTECHNOLOGY IN CROP IMPROVEMENT

Lecture 3 : Tissue Culture Media

 

Table 3.3: The composition of Murashige and Skoog (MS) Medium (1962)

  • •  Stock concentration of macronutrients is for 20 litres of medium, while micronutrients, iron and vitamins stock concentrations are prepared for 200 litres of medium.

    • •  Myoinositol and sucrose are added freshly to the medium.

    •  Dissolve 5.56 g of FeSO4 .7H2O in 350 ml of water. Apply heat if needed. Dissolve 7.46 g of Na2 EDTA in 350 ml of water. Apply heat if needed. When both solutions are dissolved, combine and bring to 1 litre final volume. The chelation reaction is forced to completion by autoclaving. The final stock solution should be deep golden yellow in color.