Module 1 : APPLICATIONS OF PLANT BIOTECHNOLOGY IN CROP IMPROVEMENT

Lecture 3 : Tissue Culture Media

 

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Table 3.1: The media elements and their functions

 

Table 3 . 2: The composition of Gamborg's B5 Medium (1968)

  • •  Stock concentration of macronutrients is for 20 litres of medium, while micronutrients stock is for 40 litres of medium, iron for 100 litres of medium and vitamins stock is for 1000 litres of medium.
  • •  Myoinositol and sucrose are added freshly to the medium.