1.4.4. Culture room

•  air (or heating / cooling system) to maintain 25±2 °C temperature

•  racks for holding test-tubes

•  lights to provide diffuse light and to maintain photoperiod

•  shakers with various sized clamps for different sized flasks to grow cells in liquid medium

•  thermostat and time clock for lights

•  wall cabinets for dark incubation of cultures

 

     

Figure 2.8: Incubator shaker and Test-tube racks

 

1.4.5. Other apparatus

•  beakers (100 mL, 250 mL, 1 L, 5 L)

•  measuring cylinders (5 mL, 10 mL, 25 mL, 50 mL, 100 mL, 500 mL, 1L, 2 L, 5 L)

•  graduated pipettes and teats

•  reagent bottles for storing liquid chemicals and stock solutions (glass or plastic)

•  culture tubes and flasks (glass or polypropylene or disposable)

•  plastic baskets

•  filter membrane, preferably nylon, of sizes 0.22 µm and 0.45 µm, holders and hypodermic syringes (for solutions requiring filter sterilization)

•  large forceps (blunt and fine points) and scalpels for dissecting and subculturing plant material.

•  Scalpel handles (no. 3) and blades (no. 11)

•  Chemicals and reagents for preparing culture media

•  Disposable gloves and masks.

•  Micropipettes of maximum volume size 5000 µL, 1000 µL, 500 µL, 250 µL, 100 µL