Module 1 : APPLICATIONS OF PLANT BIOTECHNOLOGY IN CROP IMPROVEMENT

Lecture 2 : Lab facilities and operations

    

 

Figure 2.6: Upright light microscope with CCD camera attachment

 

1.4.3. Dissection room

•  laminar-air-flow cabinet within which tissue culture work can be carried out under sterilized environment

•  glass bead sterilizer where temperature of beads is raised to 250°C in 15-20 min with 15 s cut off. Here the sterilization of instruments is effecting by pushing them into the beads for 5-7 s. This is much safer compare to the Bunsen burner heating of instruments like, forceps, needles, scalpels etc.

•  binocular microscope to observe surface details and morphogenic responses of cultures and their possible contamination.

•  low speed table-top centrifuge to sediment cells or protoplasts

 

     

Figure 2.7: Binocular stereozoom microscope (left side) and Centrifuge (right figure)