Reactions in glycolytic pathway

Glycolysis involves 10 enzymatic reactions, summarized in Figure 1:

1.  
2. 1.  The phosphorylation of glucose at position 6 by hexokinase,

2.  The isomerization of glucose-6-phosphate to fructose-6-phosphate by phosphohexose isomerase,

3. The phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate by phosphofructokinase,

4.  The cleavage of fructose-1,6-bisphosphate by aldolase. This yields two different products, dihydroxyacetone phosphate and glyceraldehyde-3-phos phate,

5.  The isomerization of dihydroxyacetone phosphate to a second molecule of glyceraldehyde phosphate by triose phosphate isomerase,

6.  The dehydrogenation and concomitant phosphorylation of glyceralde- hyde-3-phosphate to 1,3-bis-phosphoglycerate by glyceraldehyde-3-phosphate dehydrogenase,

7.  The transfer of the 1-phosphate group from 1,3-bis-phosphoglycerate to ADP by phosphoglycerate kinase, which yields ATP and 3-phosphoglycerate,

8.  The isomerization of 3-phosphoglycerate to 2-phosphoglycerate by phos phoglycerate mutase,

9.  The dehydration of 2-phosphoglycerate to phosphoenolpyruvate by eno lase.

10.  The transfer of the phosphate group from phosphoenolpyruvate to ADP by pyruvate kinase, to yield a second molecule of ATP.