Feature |
Genomic library |
cDNA library |
Sequences present |
Ideally, all genomic sequences |
Only structural genes that are transcribed |
Contents affected by : (a) Developmental stage (b) Cell type |
No No |
Yes Yes |
Features of DNA insert(s) representing a gene: (a) Size (b) Introns (c) 5´- and 3´- regulatory sequences |
As present in genome Present Present |
Ordinarily, much smaller Absent Absent |
As compared to the genome (a) Enrichment of sequences (b) Redundancy in frequency (c) Variant forms of a gene |
In amplified genomic libraries In amplified libraries Not possible |
For abundant mRNAs For rare mRNA species For such genes, whose RNA transcripts are alternatively spliced |
Table 4-4.8. Features of genomic and cDNA library
Bibliography
Brown TA. 2010. Gene cloning and DNA analysis: An Introduction. 6 th ed. Wiley-Blackwell.
Lodge J. 2007. Gene cloning: principles and applications. Taylor & Francis Group.
Moore DD. 2001. Genomic DNA Libraries. Current Protocols in Molecular Biology. DOI: 10.1002/0471142727.mb0501s00
Primrose SB, Twyman RM, Old RW. 2001. Principles of Gene Manipulation. Oxford: Blackwell Scientific.
Reece RJ. 2000. Analysis of Genes and Genomes. John Wiley & Sons, U.K.
Singh BD. 2008. Biotechnology: Expanding Horizons. 2nd ed. Kalyani Publishers.