ii. Restriction enzymes that generate sticky ends

Genomic DNA can be digested with commonly available restriction enzymes that generate sticky ends. For example, digestion of genomic DNA with the restriction enzyme Sau3AI (recognition sequence 5'-GATC-3') generates DNA fragments that are compatible with the sticky end produced by BamHI (recognition sequence 5'-GGATCC-3') cleavage of a vector. Once the DNA fragments are produced, they are cloned into a suitable vector.

Figure 4-4.2: Construction of genomic library