Fig 1-2.2.6: Preparation of competent cells

1-2.2.7 Selection of host cells/organism containing vector sequences:

Selection of the transformed cells from the non-transformed population is done by using selectable marker genes that confers resistance to antibiotics. Hence, cells only having the vector with the resistance gene for the antibiotic would grow in the selection media containing the antibiotic (ampicillin, tetracycline etc.); while the non-transformed cells would die.

Fig 1-2.2.7: Selection of transformed cells