1-2.3 Applications:

Molecular cloning serves as a tool for developing of various recombinant cells and organisms which has application both in basic and applied biological science as discussed below.

• Production of recombinant proteins: Genes encoding proteins with diagnostic, therapeutic or commercial value can be cloned, expressed and purified to obtain recombinant proteins in bulk with limited space and lesser time. Examples include Humulin – the human insulin expressed in E. coli .
  
  
• Gene therapy is used for correcting a disorder or deficiency.
  
  
• To study the structure and function of a particular gene using a model host organism.
  
  
• To study the regulation of gene expression during developmental stages.
  
  
• Complete genome sequence of an organism having large genome size can be facilitated by BAC or YAC library formation using rDNA technology.  

 

Bibliography:

• Brown T.A. 2010. Gene cloning and DNA analysis: an introduction (6^th edition); Willey Blackwell Ltd.
• Griffiths A.J.F, Miller J.H., Suzuki D.T, Lewontin R.C., Gelbert W.M. 2000. An Introduction to Genetic Analysis (7^th edition); New York: W. H. Freeman.
• Robertis E.D.P.De, Robertis E.M.F. De. 2010. Cell and Molecular biology (8^th edition); Lippincott Williams and Wilkins.