2) Eukaryotic promoter: Promoter of RNA POL l and POL ll are mostly located at upstream site, but promoter of  rRNA lll are located on downstream site of the start point. Each promoter contain some specific sequence which get recognise by the transcription factor. Eukaryotic promoter has longer region than prokaryotic promoter  because it contain all those sequences which are important regarding to initiation, it includes core promoter element at which RNA polymerase get attached and form initiation complex  and also for efficient transcription it requires an upstream promoter element, which are basically G+C rich region and at which transcription factors are bind.

TRANSCRIPTION INITIATION BY RNA POLYMERASE ǀǀ : Eukaryotic m RNA transcription requires initiation complex which consist general transcription factors (GTFs) and mediator. Various transcription factor and their functions are as follows: