Due to formation of stem loop structure, the A-U bonds get break down,  leads termination and  RNA molecules get separated

Figure 28.9(c) : Chain termination

2. Rho dependent termination:  This type of termination requires Rho protein. Rho is an ATP dependent helicase that disrupts RNA –DNA hybrids. It is an essential protein which causes transcription termination, rho protein is hexamer ATP dependent helicases and its subunit contain RNA binding and ATP hydrolysis domain. These rho protein firstly bind to sequence which is present at upstream of termination site; these sites are called rut site. These site are rich in C residues rho factor followed to RNA polymerase until it do not catch RNA polymerase, which described in Figure 28.10(a). Rho follow the RNA polymerase by its helicase activity which is driven by ATP hydrolysis ,when RNA polymerase  reached at terminator site the rho protein freeze the structure of polymerase which are given in fig 1.10(b) and when Rho factor collapse with the enzyme which cause termination and new  RNA chain get released , given in figure 1.10(c). Steps in rho-dependant termination which described in figure 1.10(a),(b),(c).

Figure 28.10(a) : Rho factor is following to RNA Polymerase