1.10.8. How to Read Enzyme Function?- Chemical Reporters Can Read
Along with monitoring biomolecule expression and localization, chemical reporters can read enzyme function. Thus, the target protein is labeled with the chemical reporter by virtue of its catalytic activity on a modified substrate. Thus, the activity-based protein profiling approach has been used to monitor enzymatic functions. An alkyne reporter was found to give cleaner labeling than the corresponding azido analog for such purpose.

1.10.9. Example of Bioorthogonal Chemical Reporters in Living Organisms
GFP-protein fusions are widely used for noninvasive imaging of protein expression and localization in living organisms. In a similar manner, both proteins and glycans have been labeled with azides in laboratory mice can be utilized for non invasive imaging.

1.10.10. Summary of Chemistry of Living Cell
As is stated earlier, the bioorthogonal chemical reporter strategy offers a means to visualize many classes of biomolecules in living systems. Substrates linked to chemical reporters can be metabolized by cells and incorporated into proteins, glycans, lipids and other cellular species. After covalent reaction with complementary probes, these classes of biomolecules can be visualized in living cells/living organisms.
Thus, it is clear that chemical reporters and bioorthogonal reactions have a rich future in the field of chemical biology. However, there remains challenge with respect to both metabolic labeling and chemical tagging in biological systems.