Laboratory experiment 35.2: Study the co-localization of two proteins in eukaryotic cell using TEM.
Material and equipment:

• All material and equipment related to the TEM experiment related to experiment 35.1.
• Primary antibody against protein-x generated in rabbit.
• Primary antibody against protein-x generated in mouse.
• Secondary antibody against rabbit coupled to 5nm gold particle.
• Secondary antibody against mouse coupled to 15nm gold particle. 

Obserrvation: A typical TEM image is given in the Figure 35.3. As you can observe that cell shows black dots with two different diameters (5 or 15nm) which indicate the presence of two proteins in the eukaryotic cell.

Figure 35.3: A typical image showing localization of two proteins in eukaryotic cell observed with TEM.  Small gold particle, 5 nm and large particle 15nm; Bar, 200 nm