Microassay:

1. In the Bradford microassay, the standard protein stock solution is prepared with a concentration of 100 μg/ml .
   
   
2. Make the dilutions of the unknown protein sample exactly as prepared in standard assay.
   
   
3. Follow steps 3 – 6 of the standard Bradford assay.
   
   
4. Add 1 ml of Bradford reagent in each of the tubes and mix well by inversion or gentle vortex mixing (avoid frothing).
   
   
5. Within 5 – 60 min , measure the absorbance of tubes 1 – 10 and 12 – 15 at 595 nm in the quartz/glass cuvette against the reagent blank (tube 11).
   
   
6. Record the readings in the observation table.

Analysis: Let us take some hypothetical readings for carrying out the analysis: