Materials:

Equipments:

1. A visible range spectrophotometer
2. Vortex mixer
3. Weighing balance

Reagents:

1. Bradford reagent
2. Ovalbumin (Protein standard)  

Glassware and plasticware:

1. Pipettes
2. Pipette tips
3. A 5 ml glass pipette
4. Pipette aid
5. 100 ml measuring cylinder
6. Test tubes (for standard assay)
7. 1.5 ml microfuge tubes (for microassay)
8. Plastic cuvettes

Preparation of reagents:

Bradford reagent : Bradford reagent is prepared as follows:

1. Weigh 200 mg of Coomassie Blue G250 dye and dissolve it in 50 ml of 95% ethanol.
2. Mix this solution with 100 ml of concentrated (85%) phosphoric acid.
3. Make the final volume of the solution to 1 litre by adding distilled water.
4. Filter the reagent through Whatman No. 1 filter paper.
5. Transfer the filtrate in an amber colored bottle and store at room temperature.

Protein standard : Ovalbumin; the standard solution is prepared as follows:

1. Weigh accurately 5 mg ovalbumin.
2. Dissolve it in 5 ml distilled water; this gives a protein stock solution of 1 mg/ml concentration.
3. Store the protein standard at –20°C.