Figure 33.2: Principle of affinity chromatography.

Different types of affinity chromatography: Affinity chromatography is further divided into the different types based on the nature of receptor present on matrix to binds tag present on the analyte molecule. Different types of affinity chromatography are-

Bio-affinity chromatography- In this type of affinity chromatography, biomolecules are used as receptor present on matrix and it exploit the biological affinity phenomenon such as antibody-antigen. In addition, enzyme-substrate or enzyme-inhibitor is also belong to this class. Ex. GST-Glutathione.   

Pseudo-affinity chromatography-In this affinity chromatography, a non-biological molecule is used as receptor on matrix to exploit the separation and purification of biomolecules. There are two specific example to this class-