In Exocarpus, an auxin (IAA) along with cytokinin (Kinetin) was required for direct shoot regeneration (Johri and Bhojwani 1965). Addition of zeatin in WM gave rise to green shoots from the intact seed (i.e. endosperm with embryo) culture of Scurrula pulverulenta which on subculture gave rise to characteristic haustoria (Bhojwani and Johri 1970). In Taxillus vestitus, shoot bud formation occurred on WM supplemented with IAA, Kinetin and CH, after seven weeks. Replacement of IAA with IBA could induce shoot regeneration in 55% cultures and haustoria in 60% cultures (Johri and Nag 1970). Here, the embryo had an adverse effect on bud differentiation from endosperm. Injury to the endosperm was found to be beneficial for shoot induction in some cases; shoot buds first develop along the injured region. The position of the explant on medium plays a significant role in regeneration of shoot in Taxillus spp. When half split T. vestitus endosperm without embryo was placed on medium with its cut surface in contact with the medium containing Kinetin, 100 % of the cultures produced shoots. In Leptomeria acida, IBA proved more efficient than IAA in terms of rapid callus proliferation. However, on IAA supplemented medium the callus gave rise to shoots in 100% cultures (Nag and Johri 1971).

The callus proliferation from endosperm and the subsequent shoot organogenesis was also reported in Jatropa panduraefolia, Putranjiva roxburghii, Codiaeum variegatum, Malus pumila, Oryza sativa, Annona squamosa, Actinidia chinensis, Mallotus philippensis, Actinidia deliciosa, Morus alba, Azadirachta indica and Actinidia deliciosa. In Actinidia species callus initiation occurred on MS medium supplemented with 2,4-D and Kinetin. Transfer of these calli to MS medium containing IAA and 2ip resulted in shoot and root organogenesis. In apple, endosperm proliferated into callus on MS medium supplemented with Kinetin + 2,4-D/BA + NAA and subsequent regeneration occurred on MS medium fortified with BA + CH. In Annona squamosa the callusing of endosperm occurred on WM supplemented with two cytokinins (Kinetin and BA), an auxin (NAA) and Gibberellic acid (GA₃). But organogenesis in the callus occurred on Nitsch's medium supplemented with BA and NAA.

In Mallotus philippensis, a continuously growing callus was obtained on MS medium supplemented with 2, 4-D + Kinetn. These calli when subcultured on MS + BA + CH gave rise to various morphologically distinct cell lines, of these, only the green compact cell line was responsive for organogenic differentiation. Shoot regeneration occurred in this callus when subcultured on MS medium fortified with BA + NAA.

In Rice, there was a striking difference in the growth response of immature and mature endosperm. Immature endosperm underwent two modes of differentition i.e. direct regeneration from the explant or indirectly via intervening callus phase. In mature endosperm, shoot organogenesis was always preceded by callusing. Callus from mature endosperm was initiated and maintained on MS + 2,4-D; shoot differentiation from callus occurred on MS + IAA + Kinetin. The proliferation of immature endosperm and occasional shoot formation occurred on YE supplemented medium; addition of IAA and Kinetin improved the response further.

Immature endosperms of neem (Azadirachta indica) showed best callusing on MS + NAA + BA + CH. When the callus was transferred to a medium containing BA or Kinetin, shoot buds differentiated from all over the callus. Maximum regeneration in terms of number of cultures showing shoot-buds and number of buds per callus cultures occurred in the presence of BA (Figure 10.1C). Thomas et al. (2000) observed maximum callusing of Mulberry endosperm on MS + BA + NAA + CH or YE. Shoot buds were emerged when the callus was transferred on a medium containing a cytokinin or a cytokinin and a - naphthaleneacetic acid (NAA). The percent response was highest on BA and NAA containing medium. However, the number of shoots per explants was maximum when TDZ alone was used.