Figure 6-5.3(a).1.3: Steps involved in AFLP (1) Genomic DNA is digested with two restriction enzymes (in this case EcoRI and MseI) (2) Ligation of specific adapters to the digested fragments at both ends 3) & 4) PCR is performed in two steps first is pre-amplification and followed by amplification. (5) This is to visualize the result of AFLP in agarose gel and detection of bandsthrough silver stainingor by using labeledprimer (i.e. radioisotope or a fluorochrome). It is indicated by a star in the figure. (Adapted and modified from: Gabriel Romero et al 2009, Genetic fingerprinting: Advancing the frontiers of crop biology research)