Figure 6-5.3(a).1.2: Steps involved in RAPD mapping: The DNA templates are used for PCR with RAPD random primers. After completion of the PCR cycles the product was visualized on agarose gel electrophoresis.

Limitations

•  RAPD provides predominantly dominant markers.

•  It is not possible to identify that whether the amplified region originated from a DNA segment locus that is heterozygous or homozygous.

•  In case of co-dominant, RAPD markers show different-sized DNA fragments after amplification from the same locus and detected very rarely.

•  It is fully dependent on PCR reaction reagents as well as cycle conditions.