Figure 5-4.3: Schematic representation of Agrobacterium- mediated transformation. Step 1: Production of signal molecules form wounded plant cell; Step 2: Recognition of signal molecules by bacterial receptors; Step 3: Attachment of Agrobacterium to plant cell; Step 4: Activation of Vir proteins which process ss-TDNA; Step 5: Formation of immature T-complex; Step 6: T-DNA transfer; Step 7: Assembly of mature T-complex and Nuclear transport; Step 8: Random T-DNA integration in the plant genome; Step 9: Expression of bacterial genes and synthesis of bacterial proteins.

(Adapted and modified from Pacurar DI, Christensen HT, Pacurar ML, Pamfil D, Botez C, Bellini C. 2011. Agrobacterium mediated transformation: From crown gall tumors to genetic transformation. Physiological and Molecular Plant Pathology, 76: 76-81)