2-1.6 Applications:

In various applications related to genetic engineering DNA is cleaved by using these restriction enzymes.

•  They are used in the process of insertion of genes into plasmid vectors during gene cloning and protein expression experiments.

•  Restriction enzymes can also be used to distinguish gene alleles by specifically recognizing single base changes in DNA known as single nucleotide polymorphisms (SNPs). This is only possible if a mutation alters the restriction site present in the allele.

•  Restriction enzymes are used for Restriction Fragment Length Polymorphism (RFLP) analysis for identifying individuals or strains of a particular species.

   

Bibliography:

•  Lodge J. 2007. Gene cloning: principles and applications. Taylor & Francis Group.

•  Primrose S. B., Twyman R. M., and Old R. W. 2001. Principles of Gene Manipulation; Oxford: Blackwell Scientific .

•  Reece R. J. 2000. Analysis of Genes and Genomes; John Wiley & Sons, U.K.