Questions:

1. What are the basic types of cell lines and which type of tissue involve in formation of cell line?
2. What are the conditions required for cell line growth?
3. What are the criteria for choosing cell lines?
4. Which type of precaution one should take during cell line maintenance?
5. Give some example of human cell lines and what are the characteristic of those cell lines?

References:

• Fresheny, I. “Culture of animal cell – A manual of basic technique and specialized application” by; chaptor-2: Biology of cultured cells; page-23
• http://www.biology online.org/dictionary/Cell_line
• http://atcc.custhelp.com/app/answers/detail/a_id/355/~/definition-of-%22cell-line%22
• http://www.biology-online.org/dictionary/Stock_culture
• http://bio.lonza.com/uploads/tx_mwaxmarketingmaterial/Lonza_TechREF_Generation_of_Stable_Cell_Lines_low_res.pdf