NPTEL :: Biotechnology - Advanced Clinical Proteomics

Modules / Lectures

Lecture 1: Genomics to Proteomics
- LECTURE-L-1
- QUIZ-L-1
- ASSIGNMENT-L-1
- HANDOUT-L-1
Lecture 2: Basics of Amino acids and Proteins
- LECTURE-L-2
- QUIZ-L-2
- ASSIGNMENTS-L-2
- HANDOUT-L-2
Lecture 3: Proteins to Proteomics
- LECTURE-L-3
- QUIZ-L-3
- ASSIGNMENTS-L-3
- HANDOUT-L-3
Lecture 4: Sample Preparation for Proteomic Analysis
- LECTURE-L-4
- QUIZ-L-4
- ASSIGNMENTS-L-4
- HANDOUT-L-4
Llecture 5: Gel?based Proteomics
- LECTURE-L-5
- QUIZ-L-5
- ASSIGNMENTS-L-5
- HANDOUT-L-5
Lecture 6: Difference In?Gel Electrophoresis for Quantitation of Protein Expression
- LECTURE-L-6
- QUIZ-L-6
- ASSIGNMENTS-L-6
- HANDOUT-L-6
Lecture 7: Gel?based Proteomic Data Analysis
- LECTURE-L-7
- QUIZ-L-7
- ASSIGNMENTS-L-7
- HANDOUT-L-7
Lecture 8: 2D?DIGE Clinical Applications
- LECTURE-L-8
- QUIZ-L-8
- ASSIGNMENTS-L-8
- HANDOUT-L-8
Lecture 9: Protein Digestion
- LECTURE-L--9
- QUIZ-L-9
- ASSIGNMENTS-L-9
- HANDOUT-L-9
Lecture 10: High Performance Liquid Chromatography Mass Spectrometry
- LECTURE-L-10
- QUIZ-L-10
- ASSIGNMENTS-L-10
- HANDOUT-L10
Lecture 11: Hybrid MS Configurations
- LECTURE-L-11
- QUIZ-L-11
- ASSIGNMENTS-L-11
- HANDOUT-L-11
Lecture 12: Tandem Mass Spectrometry for Protein Identification
- LECTURE-L-12
- QUIZ-L-12
- ASSIGNMENTS-L-12
- HANDOUT-L-12
Lecture 13: Peptide Mass Fingerprinting
- LECTURE-L-13
- QUIZ-L-13
- ASSIGNMENTS-L-13
- HANDOUT-L-13
Lecture 14: In vitro Quantitative Proteomics using iTRAQ
- LECTURE-L-14
- QUIZ-L-14
- ASSIGNMENT-L-14
- HANDOUT-L-14
Lecture 15: iTRAQ Clinical Applications
- LECTURE-L-15
- QUIZ-L-15
- ASSIGNMENTS-L-15
- HANDOUT-L-15
Lecture 16: In vivo Quantitative Proteomics using SILAC
- LECTURE-L-16
- QUIZ-L-16
- ASSIGNMENTS-L-16
- HANDOUT-L-16
Lecture 17: SILAC Clinical Applications
- LECTURE-L-17
- QUIZ-L-17
- ASSIGNMENTS-L-17
- HANDOUT-L-17
Lecture 18: Isotope?Coded Affinity Tagging (ICAT)
- LECTURE-L-18
- QUIZ-L-18
- ASSIGNMENTS-L-18
- HANDOUT-L-18
Lecture 19: MS for PTM analysis
- LECTURE-L-19
- QUIZ-L-19
- ASSIGNMENTS-L-19
- HANDOUT-L-19
Lecture 20: Mid-Semester Examination
- LECTURE-L-20
- QUIZ-L-20
- ASSIGNMENTS-L-20
- HANDOUT-L-20
Lecture 21: Interactomics-Techniques to Study Protein?Protein Interactions
- LECTURE-L-21
- QUIZ-L-21
- ASSIGNMENT-L-21
- HANDOUT-L-21
Lecture 22: Antigen and Antibody Microarrays
- LECTURE-L-22
- QUIZ-L-22
- ASSIGNMENT-L-22
- HANDOUT-L-22
Lecture 23: Reversed?Phase Protein Microarrays
- LECTURE-L-23
- QUIZ-L-23
- ASSIGNMENT-L-23
- HANDOUT-L-23
Lecture 24: Cell?free Expression Based Protein Microarrays
- LECTURE-L-24
- QUIZ-L-24
- ASSIGNMENT-L-24
- HANDOUT-L-24
Lecture 25: Nucleic Acid Programmable Protein Arrays
- LECTURE-L-25
- QUIZ-L-25
- ASSIGNMENT-L-25
- HANDOUT-L-25
Lecture 26: Microarrays for Autoantibody Profiling
- LECTURE-L-26
- QUIZ-L-26
- ASSIGNMENT-L-26
- HANDOUT-L-26
Lecture 27: Microarrays for PTM Analysis
- LECTURE-L-27
- QUIZ-L-27
- ASSIGNMENT-L-27
- HANDOUT-L-27
Lecture 28: Label?free Proteomics
- LECTURE-L-28
- QUIZ-L-28
- ASSIGNMENT-L-28
- HANDOUT-L-28
Lecture 29: Surface Plasmon Resonance
- LECTURE-L-29
- QUIZ-L-29
- ASSIGNMENT-L-29
- HANDOUT-L-29
Lecture 30: Surface Plasmon Resonance Imaging
- LECTURE-L-30
- QUIZ-L-30
- ASSIGNMENT-L-30
- HANDOUT-L-30
Lecture 31: Protein Interaction Analysis using SPR and SPRi
- LECTURE-L-31
- QUIZ-L-31
- ASSIGNMENT-L-31
- HANDOUT-L-31
Lecture 32: Nanotechnologies in Proteomics
- LECTURE-L-32
- QUIZ-L-32
- ASSIGNMENT-L-32
- HANDOUT-L-32
Lecture 33: Challenges in Clinical Proteomics
- LECTURE-L-33
- QUIZ-L-33
- ASSIGNMENT-L-33
- HANDOUT-L-33
Lecture 34: Serum Proteomics
- LECTURE-L-34
- QUIZ-L-34
- ASSIGNMENT-L-34
- HANDOUT-L-34
Lecture 35: Urine Proteomics
- LECTURE-L-35
- QUIZ-L-35
- ASSIGNMENT-L-35
- HANDOUT-L-35
Lecture 36: Salivary Proteomics
- LECTURE-L-36
- QUIZ-L-36
- ASSIGNMENT-L-36
- HANDOUT-L-36
Lecture 37: Bioinformatics and Proteomics
- LECTURE-L-37
- QUIZ-L-37
- ASSIGNMENT-L-37
- HANDOUT-L-37
Lecture 38: Proteomics for Translational Research
- LECTURE-L-38
- QUIZ-L-38
- ASSIGNMENT-L-38
- HANDOUT-L-38
Lecture 39: Future of Proteomic Technologies for Clinical Applications
- LECTURE-L-39
- QUIZ-L-39
- ASSIGNMENT-L-39
- HANDOUT-L-39
Lecture 40: Final Exam
- LECTURE-L-40
- QUIZ-L-40
- ASSIGNMENT-L-40
- HANDOUT-L-40

Web Content
Downloads

Handouts (40)
Self Evaluation (40)

Module Name	Download	Description	Download Size
Lecture 1: Genomics to Proteomics	Lecture 1	Lecture 1	176
Lecture 2: Basics of Amino acids and Proteins	Lecture 2	Lecture	212
Lecture 3: Proteins to Proteomics	Lecture 3	Lecture	148
Lecture 4: Sample Preparation for Proteomic Analysis	Lecture 4	Lecture	208
Llecture 5: Gel?based Proteomics	Lecture 5	Lecture	278
Lecture 6: Difference In?Gel Electrophoresis for Quantitation of Protein Expression	Lecture 6	Lecture	162
Lecture 7: Gel?based Proteomic Data Analysis	Lecture 7	Lecture	4505
Lecture 8: 2D?DIGE Clinical Applications	Lecture 8	Lecture	449
Lecture 9: Protein Digestion	Lecture 9	Lecture	237
Lecture 10: High Performance Liquid Chromatography Mass Spectrometry	Lecture 10	Lecture	1630
Lecture 11: Hybrid MS Configurations	Lecture 11	Lecture	781
Lecture 12: Tandem Mass Spectrometry for Protein Identification	Lecture 12	Lecture	781
Lecture 13: Peptide Mass Fingerprinting	Lecture 13	Lecture	1270
Lecture 14: In vitro Quantitative Proteomics using iTRAQ	Lecture 14	Lecture	895
Lecture 15: iTRAQ Clinical Applications	Lecture 15	Lecture	282
Lecture 16: In vivo Quantitative Proteomics using SILAC	Lecture 16	Lecture	915
Lecture 17: SILAC Clinical Applications	Lecture 17	Lecture	398
Lecture 18: Isotope?Coded Affinity Tagging (ICAT)	Lecture 18	Lecture	853
Lecture 19: MS for PTM analysis	Lecture 19	Lecture	914
Lecture 20: Mid-Semester Examination	Lecture 20	Lecture	507
Lecture 21: Interactomics-Techniques to Study Protein?Protein Interactions	Lecture 21	Lecture	1207
Lecture 22: Antigen and Antibody Microarrays	Lecture 22	Lecture	2563
Lecture 23: Reversed?Phase Protein Microarrays	Lecture 23	Lecture	1037
Lecture 24: Cell?free Expression Based Protein Microarrays	Lecture 24	Lecture	9578
Lecture 25: Nucleic Acid Programmable Protein Arrays	Lecture 25	Lecture	2878
Lecture 26: Microarrays for Autoantibody Profiling	Lecture 26	Lecture	828
Lecture 27: Microarrays for PTM Analysis	Lecture 27	Lecture	764
Lecture 28: Label?free Proteomics	Lecture 28	Lecture	669
Lecture 29: Surface Plasmon Resonance	Lecture 29	Lecture	530
Lecture 30: Surface Plasmon Resonance Imaging	Lecture 30	Lecture	695
Lecture 31: Protein Interaction Analysis using SPR and SPRi	Lecture 31	Lecture	431
Lecture 32: Nanotechnologies in Proteomics	Lecture 32	Lecture	584
Lecture 33: Challenges in Clinical Proteomics	Lecture 33	Lecture	219
Lecture 34: Serum Proteomics	Lecture 34	Lecture	596
Lecture 35: Urine Proteomics	Lecture 35	Lecture	126
Lecture 36: Salivary Proteomics	Lecture 36	Lecture	468
Lecture 37: Bioinformatics and Proteomics	Lecture 37	Lecture	4299
Lecture 38: Proteomics for Translational Research	Lecture 38	Lecture	730
Lecture 39: Future of Proteomic Technologies for Clinical Applications	Lecture 39	Lecture	128
Lecture 40: Final Exam	Lecture 40	Lecture	310

Module Name	Download	Description	Download Size
Lecture 1: Genomics to Proteomics	Quiz 1	Quiz	35
Lecture 2: Basics of Amino acids and Proteins	Quiz 2	Quiz	34
Lecture 3: Proteins to Proteomics	Quiz 3	Quiz	35
Lecture 4: Sample Preparation for Proteomic Analysis	Quiz 4	Quiz	33
Llecture 5: Gel?based Proteomics	Quiz 5	Quiz	33
Lecture 6: Difference In?Gel Electrophoresis for Quantitation of Protein Expression	Quiz 6	Quiz	35
Lecture 7: Gel?based Proteomic Data Analysis	Quiz 7	Quiz	34
Lecture 8: 2D?DIGE Clinical Applications	Quiz 8	Quiz	38
Lecture 9: Protein Digestion	Quiz 9	Quiz	35
Lecture 10: High Performance Liquid Chromatography Mass Spectrometry	Quiz 10	Quiz	35
Lecture 11: Hybrid MS Configurations	Quiz 11	Quiz	34
Lecture 12: Tandem Mass Spectrometry for Protein Identification	Quiz 12	Quiz	37
Lecture 13: Peptide Mass Fingerprinting	Quiz 13	Quiz	35
Lecture 14: In vitro Quantitative Proteomics using iTRAQ	Quiz 14	Quiz	35
Lecture 15: iTRAQ Clinical Applications	Quiz 15	Quiz	35
Lecture 16: In vivo Quantitative Proteomics using SILAC	Quiz 16	Quiz	34
Lecture 17: SILAC Clinical Applications	Quiz 17	Quiz	34
Lecture 18: Isotope?Coded Affinity Tagging (ICAT)	Quiz 18	Quiz	35
Lecture 19: MS for PTM analysis	Quiz 19	Quiz	35
Lecture 20: Mid-Semester Examination	Quiz 20	Quiz	24
Lecture 21: Interactomics-Techniques to Study Protein?Protein Interactions	Quiz 21	Quiz	35
Lecture 22: Antigen and Antibody Microarrays	Quiz 22	Quiz	35
Lecture 23: Reversed?Phase Protein Microarrays	Quiz 23	Quiz	34
Lecture 24: Cell?free Expression Based Protein Microarrays	Quiz 24	Quiz	34
Lecture 25: Nucleic Acid Programmable Protein Arrays	Quiz 25	Quiz	34
Lecture 26: Microarrays for Autoantibody Profiling	Quiz 26	Quiz	34
Lecture 27: Microarrays for PTM Analysis	Quiz 27	Quiz	35
Lecture 28: Label?free Proteomics	Quiz 28	Quiz	33
Lecture 29: Surface Plasmon Resonance	Quiz 29	Quiz	34
Lecture 30: Surface Plasmon Resonance Imaging	Quiz 30	Quiz	34
Lecture 31: Protein Interaction Analysis using SPR and SPRi	Quiz 31	Quiz	34
Lecture 32: Nanotechnologies in Proteomics	Quiz 32	Quiz	35
Lecture 33: Challenges in Clinical Proteomics	Quiz 33	Quiz	36
Lecture 34: Serum Proteomics	Quiz 34	Quiz	34
Lecture 35: Urine Proteomics	Quiz 35	Quiz	35
Lecture 36: Salivary Proteomics	Quiz 36	Quiz	32
Lecture 37: Bioinformatics and Proteomics	Quiz 37	Quiz	34
Lecture 38: Proteomics for Translational Research	Quiz 38	Quiz	34
Lecture 39: Future of Proteomic Technologies for Clinical Applications	Quiz 39	Quiz	36
Lecture 40: Final Exam	Quiz 40	Quiz	24