Figure 35.3: The typical results expected from the indirect ELISA.

 

(B) Detection of antigen in ELISA:

Background Information:  The typical setup for detection of antigen from the sample is given in the Figure 35.4. In this method, a capture antibody is used to collect the antigen from the sample. Afterwards, second antibody is used to detect antigen bound to the capture antibody.  Second antibody is directed against the antigen using a unique distinct epitope. The antibody is linked to the biotin and that can be recognized by the avidin/streptavidin-HRP complex. In the last step, peroxidase substrate is used to get a readout.

Figure 35.4: Sandwich ELISA and use of different components.