2.3.6. B. Peptide Coupling Reagent:

• N-protected carboxylic acid,
• C-protected amine
• DCC, HOBT

2.3.6.C. Why not N to C peptide synthesis?

2.3.6.D. Importance of Maintaining Stereochemical Integrity During the Coupling Step

• Number of possible stereoisomers = 2n where n= no. of chiral centers
• A peptide w/ 10 AA residues has 210 possible stereoisomers.

2.3.6.D. Proteins are:-