Determination of the TNF-α level:- The mean mean absorbance of each set of sample and subtract the background absorbance from each mean. Draw a calibration by Plotting the standard TNF-α concentration against the absorbance. A typical standard TNF-α is given in the Figure 30.6. Use a regression analysis and draw the equation. Use this equation to determine the TNF concentration of the unknown sample.

Figure 30.6: TNF-α standard Curve