Materials:

Equipments:

1. A UV/Visible spectrophotometer
2. Vortex mixer
3. Weighing balance
4. Water bath

Reagents:

1. Diphenylamine reagent
2. Calf thymus DNA
3. Glacial acetic acid
4. Concentrated sulfuric acid

Glassware and plasticware:

1. Pipettes
2. Pipette tips
3. A 5 ml glass pipette
4. Pipette aid
5. A 100 ml measuring cylinder
6. A 250 ml amber coloured glass bottle
7. Test tubes
8. Caps for glass tubes
9. Distilled water
10. Quartz or glass cuvettes

Preparation of reagents:

Diphenylamine (DPA) reagent :

1. Weigh 1g of diphenylamine and transfer it into a 250 ml amber coloured glass bottle.
2. Add 100 ml glacial acetic acid and shake well to achieve complete dissolution.
3. Add 2.5 ml of concentrated sulfuric acid.
4. Store the reagent in dark at 2 – 8°C.

Calf thymus DNA (100 μg/ml)

Prepare 100 μg/ml of calf thymus DNA solution in distilled water.