Total marks: 40 Instructions: (1) Attempt all Questions. (2) Draw the diagram as needed. |
Time allowed=2 Hrs |
Q 1: If the spectra shown below are for two proteins, A and B, identify the major secondary structural component present in these proteins:
Q 2: You are given two DNA molecules, A and B having equal lengths. Thermal denaturation curves of the two DNAs are different from each other as shown below:
- Calculate the approximate melting temperatures of the two DNA molecules. Marks=2
- What does the difference in denaturation curve suggest? Marks=2
Q 3: The figure below shows the thermal denaturation of the three related proteins A, B, and C using circular dichroism spectroscopy:
- Arrange the three proteins in the order of their increasing stability. Marks=2
- Why is CD monitored at 220 nm for protein unfolding experiments? Marks=2