Running and staining-The gel cassette is placed in the electrophoresis tank submerged completely and DNA loaded into the well with the help of pipetman and run with a constant voltage. DNA runs from negative to positive end and ethidium bromide (EtBr) present in the gel stain the DNA. Observing the agarose gel in a UV-chamber shows the DNA stained with EtBr as organe colored fluorescence (Figure 36.3).

Figure 36.3: Observation of DNA stained with EtBr in a UV chamber.

Applications of Horizontal Agarose Gel Electrophoresis: Horizontal gel electrophoresis is used to answer several biological, molecular biology and cell biology question. Here, in today’s lecture we will discuss few selected example of application of horizontal gel electrophoresis-