Gel electrophoresis:

Vertical Gel Electrophoresis: The electrophoresis in this system performed in a discontinuous way with buffer in the upper and lower tank connected by the gel slab. It has multiple modification in the running condition to answer multiple analytical questions.  

1. SDS-Polyacrylamide gel electrophoresis (SDS-PAGE)
Instrument- The schematic diagram of a vertical gel electrophoresis apparatus is given in Figure 35.2. It has two buffer chamber, upper chamber and a lower chamber. Both chamber are fitted with the platinum electrodes connected to the external power supply from a power pack which supplies a direct current or DC voltage. The upper and lower tank filled with the running buffer is connected by the electrophoresis gel casted in between two glass plates (rectangular and notched). There are additional accessories needed for casting the polyacrylamide gel such as comb (to prepare different well), spacer, gel caster etc.     

Figure 35.2: Different components of vertical gel electrophoresis apparatus.