Growth Media for yeast Expression System

Yeast expression system offers advantages of speedy growth, easy genetic manipulation, low cost media with the characteristics of higher eukaryotic systems such as post translational modifications and secretory expression. Yeast expression system mainly utilizes Saccharomyces cerevisiae (S. cerevisiae ) and Pichia pastoris ( P. pastoris ) strains for cloning and protein overexpression. For the growth, propagation and protein overexpression of these strains specific formulations and ingredients are required (Table 8.3). The common type of yeast expression media are given in Table 8.3.

Method of Preparation. Media preparation of yeast expression system is similar to the microbiology media. As per the media composition, constituents are in 950 ml of water and autoclave. Allow medium to cool to 50°C and then add 50ml of filtered sterile 40% dextrose (glucose) so that the final concentration become 2% . Adjust the final volume to 1 litre, if necessary.